Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: EST sequencing and gene expression profiling of defence-related genes from Persea americana infected with Phytophthora cinnamomi | Mahomed and van den Berg BMC Plant Biology 2011 11 167 http 1471 -2229 11 167 BMC Plant Biology RESEARCH ARTICLE Open Access EST sequencing and gene expression profiling of defence-related genes from Persea americana infected with Phytophthora cinnamomi Waheed Mahomed1 2 and Noelani van den Berg1 2 Abstract Background Avocado Persea americana belongs to the Lauraceae family and is an important commercial fruit crop in over 50 countries. The most serious pathogen affecting avocado production is Phytophthora cinnamomi which causes Phytophthora root rot PRR . Root pathogens such as P. cinnamomi and their interactions with hosts are poorly understood and despite the importance of both the avocado crop and the effect Phytophthora has on its cultivation there is a lack of molecular knowledge underpinning our understanding of defence strategies against the pathogen. In order to initiate a better understanding of host-specific defence we have generated EST data using 454 pyrosequencing and profiled nine defence-related genes from Pc-infected avocado roots. Results Mb of data was generated consisting of 10 000 reads on a single lane of the GS FLX platform. Using the Newbler assembler 371 contigs were assembled of which 367 are novel for Persea americana. Genes were classified according to Gene Ontology terms. In addition to identifying root-specific ESTs we were also able to identify and quantify the expression of nine defence-related genes that were differentially regulated in response to P. cinnamomi. Genes such as metallothionein thaumatin and the pathogenesis related PsemI mlo and profilin were found to be differentially regulated. Conclusions This is the first study in elucidating the avocado root transcriptome as well as identifying defence responses of avocado roots to the root pathogen P. cinnamomi. Our data is currently the only EST data that has been generated for avocado rootstocks and the ESTs identified in this study have already been
