Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Patterns of sequence polymorphism in the fleshless berry locus in cultivated and wild Vitis vinifera accessions | Houel et al. BMC Plant Biology 2010 10 284 http 1471-2229 10 284 BMC Plant Biology RESEARCH ARTICLE Open Access Patterns of sequence polymorphism in the fleshless berry locus in cultivated and wild Vitis vinifera accessions f I A I I I IS-. I 1D I D I I 1 2 I I I A I Av3 r I I f I 11 r A r A 1 I í DA Vr r 4 D wx A D s r I I I ZM i4 Cléa Houel Rémi Bounon Jamila Cnaib Cécile Guicnard Jean-Pierre Péros Roberto Bacilieri Alovic Doroortor4 Al irólio c lior1 THiom I 2mmho4 A miHmi I IkKPii x o4 Mnrici-rhrictinQ I o p clior2 Alexis Deieepei Auiélie Canaguiei Iiiieiiy Lacoiiiue Amidou N Diaye Maiie Ciiiisune Le rasiiei l l -s ri O Cfo K t H 3 n I o Ấ okkìokox 5 6 hlix ior f mriỳrnn5 rh it Yiif ii ii IO Rn Iiool2 p trir-o THic4 I 31 IKOKÌÍ Tr ti i orii r iCTi4 vaiie Stépnaiiie veineiey Oliviei coiiion Dominique Biunel raiiice Illis Laurent loiiegiosa Anne-Frangoise Adam-Blondon1 Abstract Background Unlike in tomato little is known about the genetic and molecular control of fleshy fruit development of perennial fruit trees like grapevine Vitis vinifera L. . Here we present the study of the sequence polymorphism in a 1 Mb grapevine genome region at the top of chromosome 18 carrying the fleshless berry mutation flb in order first to identify SNP markers closely linked to the gene and second to search for possible signatures of domestication. Results In total 62 regions 17 SSR 3 SNP 1 CAPS and 41 re-sequenced gene fragments were scanned for polymorphism along a Mb interval 85 127-3 506 060 bp at the top of the chromosome 18 in both V. vinifera cv. Chardonnay and a genotype carrying the flb mutation V. vinifera cv. Ugni Blanc mutant. A nearly complete homozygosity in Ugni Blanc wild and mutant forms and an expected high level of heterozygosity in Chardonnay were revealed. Experiments using qPCR and BAC FISH confirmed the observed homozygosity. Under the assumption that flb could be one of the genes involved into the domestication syndrome of grapevine
