Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Haplotyping, linkage mapping and expression analysis of barley genes regulated by terminal drought stress influencing seed quality | Worch et al. BMC Plant Biology 2011 11 1 http 1471-2229 11 1 BMC Plant Biology RESEARCH ARTICLE Open Access Haplotyping linkage mapping and expression analysis of barley genes regulated by terminal drought stress influencing seed quality 11 12 2 3 Sebastian Worch Kalladan Rajesh Vokkaliga T Harshavardhan Christof Pietsch Viktor Korzun Lissy Kuntze 1 1 1 1 Andreas Borner Ulrich Wobus Marion S Roder Nese Sreenivasulu Abstract Background The increasingly narrow genetic background characteristic of modern crop germplasm presents a challenge for the breeding of cultivars that require adaptation to the anticipated change in climate. Thus high priority research aims at the identification of relevant allelic variation present both in the crop itself as well as in its progenitors. This study is based on the characterization of genetic variation in barley with a view to enhancing its response to terminal drought stress. Results The expression patterns of drought regulated genes were monitored during plant ontogeny mapped and the location of these genes was incorporated into a comprehensive barley SNP linkage map. Haplotypes within a set of 17 starch biosynthesis degradation genes were defined and a particularly high level of haplotype variation was uncovered in the genes encoding sucrose synthase types I and II and starch synthase. The ability of a panel of 50 barley accessions to maintain grain starch content under terminal drought conditions was explored. Conclusion The linkage expression map is an informative resource in the context of characterizing the response of barley to drought stress. The high level of haplotype variation among starch biosynthesis degradation genes in the progenitors of cultivated barley shows that domestication and breeding have greatly eroded their allelic diversity in current elite cultivars. Prospective association analysis based on core drought-regulated genes may simplify the process of identifying favourable alleles and
