Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification of miRNAs and their target genes in developing soybean seeds by deep sequencing | Song et al. BMC Plant Biology 2011 11 5 http 1471-2229 11 5 BMC Plant Biology RESEARCH ARTICLE Open Access Identification of miRNAs and their target genes in developing soybean seeds by deep sequencing Qing-Xin Song Yun-Feng Liu Xing-Yu Hu Wan-Ke Zhang Biao Ma Shou-Yi Chen Jin-Song Zhang Abstract Background MicroRNAs miRNAs regulate gene expression by mediating gene silencing at transcriptional and post-transcriptional levels in higher plants. miRNAs and related target genes have been widely studied in model plants such as Arabidopsis and rice however the number of identified miRNAs in soybean Glycine max is limited and global identification of the related miRNA targets has not been reported in previous research. Results In our study a small RNA library and a degradome library were constructed from developing soybean seeds for deep sequencing. We identified 26 new miRNAs in soybean by bioinformatic analysis and further confirmed their expression by stem-loop RT-PCR. The miRNA star sequences of 38 known miRNAs and 8 new miRNAs were also discovered providing additional evidence for the existence of miRNAs. Through degradome sequencing 145 and 25 genes were identified as targets of annotated miRNAs and new miRNAs respectively. GO analysis indicated that many of the identified miRNA targets may function in soybean seed development. Additionally a soybean homolog of Arabidopsis SUPPRESSOR OF GENE SLIENCING 3 AtSGS3 was detected as a target of the newly identified miRNA Soy_25 suggesting the presence of feedback control of miRNA biogenesis. Conclusions We have identified large numbers of miRNAs and their related target genes through deep sequencing of a small RNA library and a degradome library. Our study provides more information about the regulatory network of miRNAs in soybean and advances our understanding of miRNA functions during seed development. Background MicroRNAs miRNAs are endogenous 21-nt noncoding RNAs derived from single-stranded RNA
