Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: The transcription factor PHR1 plays a key role in the regulation of sulfate shoot-to-root flux upon phosphate starvation in Arabidopsis | Rouached et al. BMC Plant Biology 2011 11 19 http 1471-2229 11 19 BMC Plant Biology RESEARCH ARTICLE Open Access The transcription factor PHR1 plays a key role in the regulation of sulfate shoot-to-root flux upon phosphate starvation in Arabidopsis Hatem Rouached David Secco Bulak Arpat Yves Poirier Abstract Background Sulfate and phosphate are both vital macronutrients required for plant growth and development. Despite evidence for interaction between sulfate and phosphate homeostasis no transcriptional factor has yet been identified in higher plants that affects at the gene expression and physiological levels the response to both elements. This work was aimed at examining whether PHR1 a transcription factor previously shown to participate in the regulation of genes involved in phosphate homeostasis also contributed to the regulation and activity of genes involved in sulfate inter-organ transport. Results Among the genes implicated in sulfate transport in Arabidopsis thaliana SULTR1 3 and SULTR3 4 showed up-regulation of transcripts in plants grown under phosphate-deficient conditions. The promoter of SULTR1 3 contains a motif that is potentially recognizable by PHR1. Using the phr1 mutant we showed that SULTR1 3 up-regulation following phosphate deficiency was dependent on PHR1. Furthermore transcript up-regulation was found in phosphate-deficient shoots of the phr1 mutant for SULTR2 1 and SULTR3 4 indicating that PHR1 played both a positive and negative role on the expression of genes encoding sulfate transporters. Importantly both phr1 and sultr1 3 mutants displayed a reduction in their sulfate shoot-to-root transfer capacity compared to wild-type plants under phosphate-deficient conditions. Conclusions This study reveals that PHR1 plays an important role in sulfate inter-organ transport in particular on the regulation of the SULTR1 3 gene and its impact on shoot-to-root sulfate transport in phosphate-deficient plants. PHR1 thus .
