Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Proteomic identification of OsCYP2, a rice cyclophilin that confers salt tolerance in rice (Oryza sativa L.) seedlings when overexpressed | Ruan et al. BMC Plant Biology 2011 11 34 http 1471-2229 11 34 BMC Plant Biology RESEARCH ARTICLE Open Access Proteomic identification of OsCYP2 a rice cyclophilin that confers salt tolerance in rice Oryza sativa L. seedlings when overexpressed 1 1 2 1 2 1 Song-Lin Ruan 1 Hua-Sheng Ma Shi-Heng Wang Ya-Ping Fu Ya Xin Wen-Zhen Liu Fang Wang Jian-Xin Tong1 Shu-Zhen Wang1 Hui-Zhe Chen2 Abstract Background High Salinity is a major environmental stress influencing growth and development of rice. Comparative proteomic analysis of hybrid rice shoot proteins from Shanyou 10 seedlings a salt-tolerant hybrid variety and Liangyoupeijiu seedlings a salt-sensitive hybrid variety was performed to identify new components involved in salt-stress signaling. Results Phenotypic analysis of one protein that was upregulated during salt-induced stress cyclophilin 2 OsCYP2 indicated that OsCYP2 transgenic rice seedlings had better tolerance to salt stress than did wild-type seedlings. Interestingly wild-type seedlings exhibited a marked reduction in maximal photochemical efficiency under salt stress whereas no such change was observed for OsCYP2-transgenic seedlings. OsCYP2-transgenic seedlings had lower levels of lipid peroxidation products and higher activities of antioxidant enzymes than wild-type seedlings. Spatiotemporal expression analysis of OsCYP2 showed that it could be induced by salt stress in both Shanyou 10 and Liangyoupeijiu seedlings but Shanyou 10 seedlings showed higher OsCYP2 expression levels. Moreover circadian rhythm expression of OsCYP2 in Shanyou 10 seedlings occurred earlier than in Liangyoupeijiu seedlings. Treatment with PEG heat or ABA induced OsCYP2 expression in Shanyou 10 seedlings but inhibited its expression in Liangyoupeijiu seedlings. Cold stress inhibited OsCYP2 expression in Shanyou 10 and Liangyoupeijiu seedlings. In addition OsCYP2 was strongly expressed in shoots but rarely in roots in two rice hybrid varieties. Conclusions .
