Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Seed-specific elevation of non-symbiotic hemoglobin AtHb1: beneficial effects and underlying molecular networks in Arabidopsis thaliana | Thiel et al. BMC Plant Biology 2011 11 48 http 1471-2229 11 48 BMC Plant Biology RESEARCH ARTICLE Open Access Seed-specific elevation of non-symbiotic hemoglobin AtHb1 beneficial effects and underlying molecular networks in Arabidopsis thaliana 1 1 1 2 3 2 Johannes Thiel Hardy Rolletschek Svetlana Friedel John E Lunn Thuy H Nguyen Regina Feil Henning Tschiersch1 Martin Muller1 Ljudmilla Borisjuk1 Abstract Background Seed metabolism is dynamically adjusted to oxygen availability. Processes underlying this auto-regulatory mechanism control the metabolic efficiency under changing environmental conditions stress and thus are of relevance for biotechnology. Non-symbiotic hemoglobins have been shown to be involved in scavenging of nitric oxide NO molecules which play a key role in oxygen sensing balancing in plants and animals. Steady state levels of NO are suggested to act as an integrator of energy and carbon metabolism and subsequently influence energy-demanding growth processes in plants. Results We aimed to manipulate oxygen stress perception in Arabidopsis seeds by overexpression of the non-symbiotic hemoglobin AtHbl under the control of the seed-specific LeB4 promoter. Seeds of transgenic AtHbl plants did not accumulate NO under transient hypoxic stress treatment showed higher respiratory activity and energy status compared to the wild type. Global transcript profiling of seeds siliques from wild type and transgenic plants under transient hypoxic and standard conditions using Affymetrix ATH1 chips revealed a rearrangement of transcriptional networks by AtHbl overexpression under non-stress conditions which included the induction of transcripts related to ABA synthesis and signaling receptor-like kinase- and MAP kinase-mediated signaling pathways WRKY transcription factors and ROS metabolism. Overexpression of AtHbl shifted seed metabolism to an energy-saving mode with the most prominent alterations occurring in cell wall metabolism. In .
