Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Integration of molecular biology tools for identifying promoters and genes abundantly expressed in flowers of Oncidium Gower Ramsey | Hsu et al. BMC Plant Biology 2011 11 60 http 1471-2229 11 60 BMC Plant Biology METHODOLOGY ARTICLE Open Access Integration of molecular biology tools for identifying promoters and genes abundantly expressed in flowers of Oncidium Gower Ramsey 1111 2 3 4 Chen-Tran Hsu De-Chih Liao Fu-Hui Wu Nien-Tze Liu Shu-Chen Shen Shu-Jen Chou Shu-Yun Tung Chang-Hsien Yang5 Ming-Tsair Chan1 6 t and Choun-Sea 11 Abstract Background Orchids comprise one of the largest families of flowering plants and generate commercially important flowers. However model plants such as Arabidopsis thaliana do not contain all plant genes and agronomic and horticulturally important genera and species must be individually studied. Results Several molecular biology tools were used to isolate flower-specific gene promoters from Oncidium Gower Ramsey Onc. GR . A cDNA library of reproductive tissues was used to construct a microarray in order to compare gene expression in flowers and leaves. Five genes were highly expressed in flower tissues and the subcellular locations of the corresponding proteins were identified using lip transient transformation with fluorescent protein-fusion constructs. BAC clones of the 5 genes together with 7 previously published flower- and reproductive growth-specific genes in Onc. GR were identified for cloning of their promoter regions. Interestingly 3 of the 5 novel flower-abundant genes were putative trypsin inhibitor TI genes OnTI1 OnTI2 and OnTI3 which were tandemly duplicated in the same BAC clone. Their promoters were identified using transient GUS reporter gene transformation and stable A. thaliana transformation analyses. Conclusions By combining cDNA microarray BAC library and bombardment assay techniques we successfully identified flower-directed orchid genes and promoters. Background The Orchidaceae family comprises an estimated 35 000 species and is one of the largest families of flowering plants. The Oncidiinae subtribe consists of 70 .
