Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Introgression of Swertia mussotii gene into Bupleurum scorzonerifolium via somatic hybridization | Wang et al. BMC Plant Biology 2011 11 71 http 1471-2229 11 71 BMC Plant Biology RESEARCH ARTICLE Open Access Introgression of Swertia mussotii gene into Bupleurum scorzonerifolium via somatic hybridization Junfeng Wang1 2 Cuizhu Zhao1 Chang Liu1 Guangmin Xia1 and Fengning Xiang1 Abstract Background The wild herb Swertia mussotii is a source of the anti-hepatitis compounds swertiamarin mangiferin and gentiopicroside. Its over-exploitation has raised the priority of producing these compounds heterologously. Somatic hybridization represents a novel approach for introgressing Swertia mussotii genes into a less endangered species. Results Protoplasts derived from calli of Bupleurum scorzonerifolium and S. mussotii were fused to produce 194 putative hybrid cell lines of which three all derived from fusions where the S. mussotii protoplasts were pretreated for 30 s with UV light later differentiated into green plants. The hybridity of the calli was confirmed by a combination of isozyme RAPD and chromosomal analysis. The hybrid calli genomes were predominantly B. scorzonerifolium. GISH analysis of mitotic chromosomes confirmed that the irradiation of donor protoplasts increased the frequency of chromosome elimination and fragmentation. RFLP analysis of organellar DNA revealed that mitochondrial and chloroplast DNA of both parents coexisted and recombined in some hybrid cell lines. Some of the hybrid calli contained SmG10H from donor and produced swertiamarin mangiferin and certain volatile compounds characteristic of S. mussotii. The expression of SmG10H geraniol 10-hydroxylase was associated with the heterologous accumulation of swertiamarin. Conclusions Somatic hybrids between B. scorzonerifolium and S. mussotii were obtained hybrids selected all contained introgressed nuclear and cytoplasmic DNA from S. mussotii and some produced more mangiferin than the donor itself. The introgression of SmG10H was necessary for the accumulation of swertiamarin. .
