Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification and characterization of plant Haspin kinase as a histone H3 threonine kinase | Kurihara et al. BMC Plant Biology 2011 11 73 http 1471-2229 11 73 BMC Plant Biology RESEARCH ARTICLE Open Access Identification and characterization of plant Haspin kinase as a histone H3 threonine kinase Daisuke Kurihara1 2 Sachihiro Matsunaga3 4 Tomohiro Omura3 Tetsuya Higashiyama1 2 and Kiichi Fukui3 Abstract Background Haspin kinases are mitotic kinases that are well-conserved from yeast to human. Human Haspin is a histone H3 Thr3 kinase that has important roles in chromosome cohesion during mitosis. Moreover phosphorylation of histone H3 at Thr3 by Haspin in fission yeast Xenopus and human is required for accumulation of Aurora B on the centromere and the subsequent activation of Aurora B kinase activity for accurate chromosome alignment and segregation. Although extensive analyses of Haspin have been carried out in yeast and animals the function of Haspin in organogenesis remains unclear. Results Here we identified a Haspin kinase designated AtHaspin in Arabidopsis thaliana. The purified AtHaspin phosphorylated histone H3 at both Thr3 and Thr11 in vitro. Live imaging of AtHaspin-tdTomato and GFP-a-tubulin in BY-2 cells showed that AtHaspin-tdTomato localized on chromosomes during prometaphase and metaphase and around the cell plate during cytokinesis. This localization of AtHaspin overlapped with that of phosphorylated Thr3 and Thr11 of histone H3 in BY-2 cells. AtHaspin-GFP driven by the native promoter was expressed in root meristems shoot meristems floral meristems and throughout the whole embryo at stages of high cell division. Overexpression of a kinase domain mutant of AtHaspin decreased the size of the root meristem which delayed root growth. Conclusions Our results indicated that the Haspin kinase is a histone H3 threonine kinase in A. thaliana. AtHaspin phosphorylated histone H3 at both Thr3 and Thr11 in vitro. The expression and dominant-negative analysis showed that AtHaspin may have a role in mitotic cell division during .
