Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Staging of biliary atresia at diagnosis by molecular profiling of the liver | Moyer et al. Genome Medicine 2010 2 33 http content 2 5 33 Genome Medicine RESEARCH Open Access Staging of biliary atresia at diagnosis by molecular profiling of the liver Katie Moyer 1 Vivek Kaimal6 Cristina Pacheco2 5 Reena Mourya1 Huan Xu1 Pranavkumar Shivakumar1 Ranajit Chakraborty3 Marepalli Rao3 John C Magee4 Kevin Bove2 Bruce J Aronow6 Anil G Jegga6 and Jorge A Bezerra 1 Abstract Background Young age at portoenterostomy has been linked to improved outcome in biliary atresia but pre-existing biological factors may influence the rate of disease progression. In this study we aimed to determine whether molecular profiling of the liver identifies stages of disease at diagnosis. Methods We examined liver biopsies from 47 infants with biliary atresia enrolled in a prospective observational study. Biopsies were scored for inflammation and fibrosis used for gene expression profiles and tested for association with indicators of disease severity response to surgery and survival at 2 years. Results Fourteen of 47 livers displayed predominant histological features of inflammation N 9 or fibrosis N 5 with the remainder showing similar levels of both simultaneously. By differential profiling of gene expression the 14 livers had a unique molecular signature containing 150 gene probes. Applying prediction analysis models the probes classified 29 of the remaining 33 livers into inflammation or fibrosis. Molecular classification into the two groups was validated by the findings of increased hepatic population of lymphocyte subsets or tissue accumulation of matrix substrates. The groups had no association with traditional markers of liver injury or function response to surgery or complications of cirrhosis. However infants with an inflammation signature were younger while those with a fibrosis signature had decreased transplant-free survival. Conclusions Molecular profiling at diagnosis of biliary atresia uncovers a signature of inflammation or fibrosis in .
