Microvascular Research biology and pathology - part 10

Một đánh giá tốt tập trung vào các nguyên tắc chung của công nghệ microarray cDNA. Evans, SJ, Datson, NA, Kabbaj, M., Thompson, RC, Vreugdenhil, E., De Kloet, ER, Watson, SJ, và Akil, H. (2002). Đánh giá của Affymetrix nhạy cảm Chip gen trong mô chuột vùng đồi thị bằng cách sử dụng phân tích | 1068 Part V New Research Modes and Procedures microarray systems can be expected to invigorate research into the microvasculature for many years to come. Glossary Expression profiling Using gene microarray or gene chip technology to assess the overall transcriptional state of a cell or tissue. Gene chip A silicon or glass slide upon which cDNA or oligonucleotides have been placed also known as a microarray. References 1. Kim et al. 2001 . Microvasc. Res. 62 83-93. 2. Bussolati et al. 2003 . FASEB J. 17 1159-1161. 3. Schmid et al. 2003 . Int. J. Cancer 103 577-586. 4. Li et al. 2002 . Circulation 105 1037-1043. 5. Poole et al. 2002 . J. Virol. 76 3395-3420. 6. Weston et al. 2002 . Mol. Hum. Reprod. 8 855-863. Bibliography Cheung V. G. Morley M. Aguilar F. Massimi A. Kucherlapati R. and Childs G. 1999 . Making and reading microarrays. Nat. Genet. 21S 15-19. Duggan D. J. Bittner M. Chen Y. Meltzer p. and Trent J. M. 1999 . Expression profiling using cDNAmicroarrays. Nat. Genet. 21S 10-14. A good review focusing on the general principles of cDNA microarray technology. Evans S. J. Datson N. A. Kabbaj M. Thompson R. C. Vreugdenhil E. De Kloet E. R. Watson S. J. and Akil H. 2002 . Evaluation of Affymetrix Gene Chip sensitivity in rat hippocampal tissue using SAGE analysis. Serial Analysis of Gene Expression. Eur. J. Neurosci. 16 409-413. Ferea T. L. Botstein D. Brown p. O. and Rosenzweig R. F. 1999 . Systematic changes in gene expression patterns following adaptive evolution in yeast. Proc. Natl. Acad. Sci. USA 96 9721-9726. Gaasterlaand T. and Baikaranov S. 2000 . Making the most of microarray data. Nat. Genet. 24 204-206. A concise review highlighting the latest advances in computational analysis of microarray data. Lander E. S. 1999 . Array of hope. Nat. Genet. 21S 3-4. A well written review on the strengths and limitations of microarray technology from one of the pioneers of the human genome project. Capsule Biography Dr. Leask is currently Visiting Lecturer in the .