Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: In vitro analysis of the cytotoxicity and the antimicrobial effect of four endodontic sealers | Willershausen et al. Head Face Medicine 2011 7 15 http content 7 1 15 HEAD FACE MEDICINE RESEARCH Open Access In vitro analysis of the cytotoxicity and the antimicrobial effect of four endodontic sealers Ines Willershausen Angelika Callaway Benjamin Briseno and Brita Willershausen Abstract Introduction The aim of this study was to investigate in vitro the cytotoxicity and antibacterial properties of four different endodontic sealers using human periodontal ligament fibroblast cell proliferation and visual analysis of growth inhibition. Methods A silicone GuttaFlow silicate EndoSequence BC zinc oxide eugenol Pulp Canal Sealer EWT and epoxy resin AH Plus Jet based sealer were incubated with PDL fibroblasts 104 cells ml n 6 up to 96 h. Cell proliferation RFU was determined by means of the Alamar Blue assay. Cell growth and morphology was visualized by means of fluorescent dyes. Possible antibacterial properties of the different sealers were visualized by means of SEM Enterococcus faecalis Parvimonas micra . Results Fibroblast proliferation depended on sealer and cultivation time. After 72 and 96 h GuttaFlow and EndoSequence BC showed relatively non-cytotoxic reactions while Pulp Canal Sealer EWT and AH Plus Jet caused a significant decrease of cell proliferation p . Visualization of cell growth and morphology with various fluorescent dyes supplemented the results. No antibacterial effect of EndoSequence BC to P. micra was found whereas GuttaFlow showed a weak Pulp Canal Sealer EWT and AH Plus Jet extensive growth inhibition. Also no antibacterial effect of GuttaFlow EndoSequence BC or AH Plus Jet to E. faecalis could be detected. Conclusions These in vitro findings reveal that GuttaFlow and EndoSequence BC can be considered as biocompatible sealing materials. However prior to their clinical employment studies regarding their sealing properties also need to be considered. Keywords in vitro study root canal sealer E. faecalis P. micra .
