Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Pepper leaf curl Lahore virus requires the DNA B component of Tomato leaf curl New Delhi virus to cause leaf curl symptoms | Shafiq et al. Virology Journal 2010 7 367 http content 7 1 367 J VIROLOGY JOURNAL RESEARCH Open Access Pepper leaf curl Lahore virus requires the DNA B component of Tomato leaf curl New Delhi virus to cause leaf curl symptoms Muhammad Shafiq Shaheen Asad Yusuf Zafar Rob W Briddon Shahid Mansoor Abstract Background Begomoviruses are whitefly-transmitted geminiviruses with genomes that consist of either two components known as DNA A and DNA B or a single component homologous to the DNA A component of bipartite begomoviruses . Monopartite begomoviruses are often associated with a symptom-modulating DNA satellite collectively known as betasatellites . Both bipartite and monopartite begomoviruses with associated satellites have previously been identified in chillies showing leaf curl symptoms in Pakistan. Results A chilli plant Capsicum annum with chilli leaf curl disease symptoms was found to contain a begomovirus a betasatellite and the DNA B component of Tomato leaf curl New Delhi virus ToLCNDV . The begomovirus consisted of 2747 nucleotides and had the highest sequence identity 99 with Pepper leaf curl Lahore virus PepLCLV- PK Lah 04 acc. no. AM404179 . Agrobacterium-mediated inoculation of the clone to Nicotiana benthamiana induced very mild symptoms and low levels of viral DNA detected in systemically infected leaves by PCR. No symptoms were induced in Nicotiana tabacum or chillies either in the presence or absence of a betasatellite. However inoculation of PepLCLV with the DNA B component of ToLCNDV induced leaf curl symptoms in N. benthamiana N. tabacum and chillies and viral DNA accumulated to higher levels in comparison to plants infected with just PepLCLV. Conclusions Based on our previous efforts aimed at understanding of diversity of begomoviruses associated with chillies we propose that PepLCLV was recently mobilized into chillies upon its interaction with DNA B of ToLCNDV. Interestingly the putative rep-binding iterons found on PepLCLV .