Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Rapid detection of porcine circovirus type 2 using a TaqMan-based real-time PCR | Zhao et al. Virology Journal 2010 7 374 http content 7 1 374 J VIROLOGY JOURNAL SHORT REPORT Open Access Rapid detection of porcine circovirus type 2 using a TaqMan-based real-time PCR 4 z 7 23 13 2 3 z z 4 23 13 Kai Zhao Fangting Han Yong Zou Lianlong Zhu Chunhua Li Yan Xu Chunling Zhang Furong Tan Jinbin Wang1 3 Shiru Tao1 3 Xizhong He2 3 Zongqing Zhou2 3 Xueming Tang1 3 Abstract Porcine circovirus type 2 PCV2 and the associated disease postweaning multisystemic wasting syndrome PMWS have caused heavy losses in global agriculture in recent decades. Rapid detection of PCV2 is very important for the effective prophylaxis and treatment of PMWS. To establish a sensitive specific assay for the detection and quantitation of PCV2 we designed and synthesized specific primers and a probe in the open reading frame 2. The assay had a wide dynamic range with excellent linearity and reliable reproducibility and detected between 102 and 1010 copies of the genomic DNA per reaction. The coefficient of variation for Ct values varied from to in the same assay and from to in 10 different assays. The assay did not cross-react with porcine circovirus type 1 porcine reproductive and respiratory porcine epidemic diarrhea transmissible gastroenteritis of pigs and rotavirus. The limits of detection and quantitation were 10 and 100 copies respectively. Using the established real-time PCR system 39 of the 40 samples we tested were detected as positive. Introduction Porcine circovirus type 2 PCV2 is widespread in the commercial swine population 1-5 and is accepted as the causative agent of a number of diseases in these animals particularly postweaning multisystemic wasting syndrome PMWS 6 . To date PCV2 infection is common in some regions of China 7 and is considered as a major problem in pig production. There is therefore an urgent need for specific and effective methods to detect the virus. By comparison with conventional PCR and ELISA real-time PCR
