Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Prime immunization with rotavirus VLP 2/6 followed by boosting with an adenovirus expressing VP6 induces protective immunization against rotavirus in mice | Zhou et al. Virology Journal 2011 8 3 http content 8 1 3 J VIROLOGY JOURNAL RESEARCH Open Access Prime immunization with rotavirus VLP 2 6 followed by boosting with an adenovirus expressing VP6 induces protective immunization against rotavirus in mice 1 1 2 2 Hongli Zhou Li Guo Min Wang Jianguo Qu Zhendong Zhao Jianwei Wang Tao Hung Abstract Background Rotavirus RV is the main cause of severe gastroenteritis in children. An effective vaccination regime against RV can substantially reduce morbidity and mortality. Previous studies have demonstrated the efficacy of virus-like particles formed by RV VP2 and VP6 VLP2 6 as well as that of recombinant adenovirus expressing RV VP6 rAd in eliciting protective immunities against RV. However the efficacy of such prime-boost strategy which incorporates VLP and rAd in inducing protective immunities against RV has not been addressed. We assessed the immune effects of different regimens in mice including rAd prime-VLP2 6 boost rAd VLP VLP2 6 prime-rAd boost VLP rAd rAd alone and VLP alone. Results Mice immunized with the VLP rAd regimen elicit stronger humoral mucosal and cellular immune responses than those immunized with other regimens. RV challenging experiments showed that the highest reduction in viral shedding was achieved in the VLP rAd group when compared with rAd VLP 25 VLP alone 75 or rAd alone 40 treatment groups. The reduction in RV shedding in mice correlated with fecal IgG r P and IgA r P . Conclusions A VLP2 6 prime-rAd boost regimen is effective in conferring immunoprotection against RV challenge in mice. This finding may lay the groundwork for an alternative strategy in novel RV vaccine development. Background Rotavirus RV infection is the most common cause of severe gastroenteritis in children. RV-induced gastroenteritis is responsible for over 600 000 deaths of children every year 85 of these deaths occur in developing countries where nearly .
