Báo cáo y học: " Comparative evaluation of the performance of the Abbott RealTime HIV-1 assay for measurement of HIV-1 plasma viral load on genetically diverse samples from Greece"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Comparative evaluation of the performance of the Abbott RealTime HIV-1 assay for measurement of HIV-1 plasma viral load on genetically diverse samples from Greece | Katsoulidou et al. Virology Journal 2011 8 10 http content 8 1 10 J VIROLOGY JOURNAL RESEARCH Open Access Comparative evaluation of the performance of the Abbott RealTime HIV-1 assay for measurement of HIV-1 plasma viral load on genetically diverse samples from Greece Antigoni Katsoulidou Chrysoula Rokka Catherine Issaris Catherine Haida Kimon Tzannis Vana Sypsa Maria Detsika Dimitrios Paraskevis Angelos Hatzakis Abstract Background HIV-1 is characterized by increased genetic heterogeneity which tends to hinder the reliability of detection and accuracy of HIV-1 RNA quantitation assays. Methods In this study the Abbott RealTime HIV-1 Abbott RealTime assay was compared to the Roche Cobas TaqMan HIV-1 Cobas TaqMan and the Siemens Versant HIV-1 RNA bDNA assays using clinical samples of various viral load levels and subtypes from Greece where the recent epidemiology of HIV-1 infection has been characterized by increasing genetic diversity and a marked increase in subtype A genetic strains among newly diagnosed infections. Results A high correlation was observed between the quantitative results obtained by the Abbott RealTime and the Cobas TaqMan assays. Viral load values quantified by the Abbott RealTime were on average lower than those obtained by the Cobas TaqMan with a mean SD difference log10 copies ml. The mean differences according to HIV-1 subtypes between the two techniques for samples of subtype A B and non-A non-B were and log10 copies ml respectively. Overall differences were less than log10 for 85 of the samples and 1 log10 in only one subtype B sample. Similarly Abbott RealTime and bDNA assays yielded a very good correlation of quantitative results whereas viral load values assessed by the Abbott RealTime were on average higher mean SD difference log10 copies ml . The mean differences according to HIV-1 subtypes between the two techniques for subtype A B and non-A non-B samples

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