Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Short distance movement of genomic negative strands in a host and nonhost for Sugarcane mosaic virus (SCMV) | Chaves-Bedoya et al. Virology Journal 2011 8 15 http content 8 1 15 J VIROLOGY JOURNAL RESEARCH Open Access Short distance movement of genomic negative strands in a host and nonhost for Sugarcane mosaic virus SCMV 1 1 1 Giovanni Chaves-Bedoya Fulgencio Espejel Ricardo I Alcala-Briseno Juan Hernández-Vela Laura Silva-Rosales1 Abstract Background In order to obtain an initial and preliminary understanding of host and nonhost resistance in the initial step of potyvirus replication both positive and negative Sugarcane mosaic virus SCMV strands where traced in inoculated and systemic leaves in host and nonhost resistant maize and sugarcane for one Mexican potyviral isolate SCMV-VER1 . Intermediary replication forms such as the negative viral strand seem to only move a short distance as surveyed by RT-PCR analysis and ELISA in different leaves. Virus purification was also done in leaves and stems. Results Susceptible maize plants allowed for viral SCMV replication cell-to-cell and long distance movement as indicated by the presence of the coat protein along the plant. In the host resistant maize plants for the SCMV-VER1 isolate the virus was able to establish the disease though the initial steps of virus replication as detected by the presence of negative strands in the basal area of the inoculated leaves at six and twelve days post inoculation. The nonhost sugarcane for SCMV-VER1 and the host sugarcane for SCMV-CAM6 also allowed the initial steps of viral replication for the VER1 isolate in the local inoculated leaf. SCMV-VER1 virions could be extracted from stems of susceptible maize with higher titers than leaves. Conclusion Nonhost and host resistance allow the initial steps of potyvirus SCMV replication as shown by the negative strands presence. Furthermore both hosts allow the negative viral strands local movement but not their systemic spread through the stem. The presence of larger amounts of extractable virions from the stem as compared to
