Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Differential cytopathogenesis of respiratory syncytial virus prototypic and clinical isolates in primary pediatric bronchial epithelial cells | Villenave et al. Virology Journal 2011 8 43 http content 8 1 43 J VIROLOGY JOURNAL RESEARCH Open Access Differential cytopathogenesis of respiratory syncytial virus prototypic and clinical isolates in primary pediatric bronchial epithelial cells 11 111 Rémi Villenave Dara O Donoghue Surendran Thavagnanam Olivier Touzelet Grzegorz Skibinski 1 2 3 1 Liam G Heaney James P McKaigue Peter V Coyle Michael D Shields Ultan F Power Abstract Background Human respiratory syncytial virus RSV causes severe respiratory disease in infants. Airway epithelial cells are the principle targets of RSV infection. However the mechanisms by which it causes disease are poorly understood. Most RSV pathogenesis data are derived using laboratory-adapted prototypic strains. We hypothesized that such strains may be poorly representative of recent clinical isolates in terms of virus host interactions in primary human bronchial epithelial cells PBECs . Methods To address this hypothesis we isolated three RSV strains from infants hospitalized with bronchiolitis and compared them with the prototypic RSV A2 in terms of cytopathology virus growth kinetics and chemokine secretion in infected PBEC monolayers. Results RSV A2 rapidly obliterated the PBECs whereas the clinical isolates caused much less cytopathology. Concomitantly RSV A2 also grew faster and to higher titers in PBECs. Furthermore dramatically increased secretion of IP-10 and RANTES was evident following A2 infection compared with the clinical isolates. Conclusions The prototypic RSV strain A2 is poorly representative of recent clinical isolates in terms of cytopathogenicity viral growth kinetics and pro-inflammatory responses induced following infection of PBEC monolayers. Thus the choice of RSV strain may have important implications for future RSV pathogenesis studies. Introduction Respiratory syncytial virus RSV infection is one of the leading causes of infant hospitalization. Virtually all children are infected by
