Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Failure to detect Xenotropic murine leukaemia virus-related virus in Chinese patients with chronic fatigue syndrome | Hong et al. Virology Journal 2010 7 224 http content 7 1 224 VIROLOGY JOURNAL RESEARCH Open Access Failure to detect Xenotropic murine leukaemia virus-related virus in Chinese patients with chronic fatigue syndrome Ping Hong1 2 Jinming Li1 2 Yongzhe Li 3 Abstract Background Recent controversy has surrounded the question of whether xenotropic murine leukaemia virus-related virus XMRV contributes to the pathogenesis of chronic fatigue syndrome CFS . To investigate the question in a Chinese population 65 CFS patients and 85 blood donor controls were enrolled and multiplex realtime PCR or reverse transcriptase PCR RT-PCR was developed to analyze the XMRV infection status of the study participants. The assay was standardized by constructing plasmid DNAs and armored RNAs as XMRV standards and competitive internal controls CICs respectively. Results The sensitivities of the multiplex real-time PCR and RT-PCR assays were 20 copies reaction and 10 IU ml respectively with 100 specificity. The within-run precision coefficient of variation CV ranged from to and to while the between-run CV ranged from to and to . XMRV was not detected in the 65 CFS patients and 65 normal individuals out of 85 controls. Conclusions This study failed to show XMRV in peripheral blood mononuclear cells PBMCs and plasma of Chinese patients with CFS. The absence of XMRV nucleic acids does not support an association between XMRV infection and the development of CFS in Chinese. Background Chronic fatigue syndrome CFS is a multisystem disease which is characterized by severe and debilitating fatigue abnormal sleep behaviour impaired memory and concentration and musculoskeletal pain 1 . The constellation of symptoms is non-specific and can be caused by a variety of diseases. Studies have identified various features relevant to the pathogenesis of CFS such as viral infection abnormal immune function exposure to toxins chemicals and pesticides .
