Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Phylogenetic history demonstrates two different lineages of dengue type 1 virus in Colombia | Mendez et al. Virology Journal 2010 7 226 http content 7 1 226 VIROLOGY JOURNAL RESEARCH Open Access Phylogenetic history demonstrates two different lineages of dengue type 1 virus in Colombia I I r ỉ h I Z l 7 1 4I r o ỉ I I f m I vr 2 k r f- I Ch m I z t z 3 5 I rỉ I Dz x 1 11 I I A c r n z 74 A t Z I z I r r I r 3 Jairo A Mendez Jose A usme-Ciro Cristina Domingo Gloria J ney Juan A Sanchez Antonio lenorio Juan C Gallego-Gomez2 Abstract Background Dengue Fever is one of the most important viral re-emergent diseases affecting about 50 million people around the world especially in tropical and sub-tropical countries. In Colombia the virus was first detected in the earliest 70 s when the disease became a major public health concern. Since then all four serotypes of the virus have been reported. Although most of the huge outbreaks reported in this country have involved dengue virus serotype 1 DENV-1 there are not studies about its origin genetic diversity and distribution. Results We used 224 bp corresponding to the carboxyl terminus of envelope E gene from 74 Colombian isolates in order to reconstruct phylogenetic relationships and to estimate time divergences. Analyzed DENV-1 Colombian isolates belonged to the formerly defined genotype V. Only one virus isolate was clasified in the genotype I likely representing a sole introduction that did not spread. lhe oldest strains were closely related to those detected for the first time in America in 1977 from the Caribbean and were detected for two years until their disappearance about six years later. Around 1987 a split up generated 2 lineages that have been evolving separately although not major aminoacid changes in the analyzed region were found. Conclusion DENV-1 has been circulating since 1978 in Colombia. Yet the phylogenetic relationships between strains isolated along the covered period of time suggests that viral strains detected in some years although belonging to the same genotype V have .
