Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Production, purification and characterization of polyclonal antibody against the truncated gK of the duck enteritis virus | Zhang et al. Virology Journal 2010 7 241 http content 7 1 241 VIROLOGY JOURNAL SHORT REPORT Open Access Production purification and characterization of polyclonal antibody against the truncated gK of the duck enteritis virus 1t 1t 1 1 w Z-I 2 Shunchuan Zhang Jun Xiang Anchun Cheng Mingshu Wang Xin Li Lijuan Li Xiwen Chen Dekang Zhu1 2 Qihui Luo2 Xiaoyue Chen1 2 3 Abstract Duck virus enteritis DVE is an acute contagious herpesvirus infection of ducks geese and swans which has produced significant economic losses in domestic and wild waterfowl. With the purpose of decreasing economic losses in the commercial duck industry studying the unknown glycoprotein K gK of DEV may be a new method for preferably preventing and curing this disease. So this is the first time to product and purify the rabbit anti-tgK polyclonal antibody. Through the western blot and ELISA assay the truncated glycoprotein K tgK has good antigenicity also the antibody possesses high specificity and affinity. Meanwhile the rabbit anti-tgK polyclonal antibody has the potential to produce subunit vaccines and the functions of neutralizing DEV and anti-DEV infection because of its neutralization titer. Indirect immunofluorescent microscopy using the purified rabbit anti-tgK polyclonal antibody as diagnostic antibody was susceptive to detect a small quantity of antigen in tissues or cells. This approach also provides effective experimental technology for epidemiological investigation and retrospective diagnose of the preservative paraffin blocks. Findings Duck virus enteritis DVE is an acute contagious herpesvirus infection of ducks geese and swans characterized by vascular damage tissue hemorrhages digestive mucosal eruptions lesions of lymphoid organs and degenerative changes in parenchymatous organs 1-5 . The causative agent of DVE is duck enteritis virus DEV composing of a linear double-stranded DNA genome with guanine-plus-cytosine content which is higher than any .
