Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Integration of bovine herpesvirus 4 genome into cultured persistently infected host cell genome | Donofrio et al. Virology Journal 2010 7 246 http content 7 1 246 VIROLOGY JOURNAL SHORT REPORT Open Access Integration of bovine herpesvirus 4 genome into cultured persistently infected host cell genome 1 1 1 2 3 Gaetano Donofrio Antonio Capocefalo Valentina Franceschi Lisa De Lorenzi Vicky van Santen Pietro Parma2 Abstract Persistent infection of macrophages with bovine herpesvirus 4 BoHV-4 has been proposed to play a secondary causal role along with bacterial infection in bovine post-partum metritis. Mechanisms of maintenance of BoHV-4 persistent infection are not understood. We previously generated in vitro models of BoHV-4 persistent infection in human rhadomyosarcoma and bovine macrophage cell lines by drug selection of cells infected with BoHV-4 carrying a drug-resistance marker and demonstrated circular episomal BoHV-4 genomes. In the present study we used fluorescent in situ hybridization FISH to demonstrate BoHV-4 genomes also integrated into the genomes of these persistently infected cells. Findings Bovine herpesvirus 4 BoHV-4 a member of the Gammaherpesvirinae subfamily was first isolated in Europe from respiratory and ocular diseases by Bartha and colleagues 1 and later in the United States by Mohanty and colleagues 2 . BoHV-4 has been isolated from a variety of samples and cells from healthy cattle and from cattle with abortion metritis pneumonia diarrhea respiratory infection and mammary pustular dermatitis 3 . However only a few investigators have successfully produced experimental disease. Although no clear direct dise ase associations have been demonstrated abundant evidence consistent with a secondary role for persistent infection by BoHV-4 in bovine postpartum metritis has accumulated 4 . Like other herpesviruses BoHV-4 establishes persistent infections in its natural host 5 6 and in an experimental host the rabbit 7 . Although BoHV-4 has been demonstrated in many tissues accumulated evidence suggests that the main site of .
