Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification and characterization of a virus-specific continuous B-cell epitope on the PrM/M protein of Japanese Encephalitis Virus: potential application in the detection of antibodies to distinguish Japanese Encephalitis Virus infection from West Nile Virus and Dengue Virus infections | Hua et al. Virology Journal 2010 7 249 http content 7 1 249 VIROLOGY JOURNAL RESEARCH Open Access Identification and characterization of a virus-specific continuous B-cell epitope on the PrM M protein of Japanese Encephalitis Virus potential application in the detection of antibodies to distinguish Japanese Encephalitis Virus infection from West Nile Virus and Dengue Virus infections 1 1 1 2 2 1 1 1 Rong-Hong Hua Na-Sha Chen Cheng-Feng Qin Yong-Qiang Deng Jin-Ying Ge Xi-Jun Wang Zu-Jian Qiao Wei-Ye Chen1 Zhi-Yuan Wen1 Wen-Xin Liu1 Sen Hu1 Zhi-Gao Bu1 Abstract Background Differential diagnose of Japanese encephalitis virus JEV infection from other flavivirus especially West Nile virus WNV and Dengue virus DV infection was greatly hindered for the serological cross-reactive. Virus specific epitopes could benefit for developing JEV specific antibodies detection methods. To identify the JEV specific epitopes we fully mapped and characterized the continuous B-cell epitope of the PrM M protein of JEV. Results To map the epitopes on the PrM M protein we designed a set of 20 partially overlapping fragments spanning the whole PrM fused them with GST and expressed them in an expression vector. Linear epitope M14 105VNKKEAWLDSTKATRY120 was detected by enzyme-linked immunosorbent assay ELISA . By removing amino acid residues individually from the carboxy and amino terminal of peptide M14 we confirmed that the minimal unit of the linear epitope of PrM M was M14-13 108KEAWLDSTKAT118 . This epitope was highly conserved across different JEV strains. Moreover this epitope did not cross-react with WNV-positive and DENV-positive sera. Conclusion Epitope M14-13 was a JEV specific lineal B-cell epitpe. The results may provide a useful basis for the development of epitope-based virus specific diagnostic clinical techniques. Background Japanese encephalitis virus JEV is the most important cause of epidemic encephalitis in most Asian regions. The virus belongs to the .
