Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Comparative study between the Hybrid Capture II test and PCR based assay for the detection of human papillomavirus DNA in oral submucous fibrosis and oral squamous cell carcinoma | Chaudhary et al. Virology Journal 2010 7 253 http content 7 1 253 VIROLOGY JOURNAL RESEARCH Open Access Comparative study between the Hybrid Capture II test and PCR based assay for the detection of human papillomavirus DNA in oral submucous fibrosis and oral squamous cell carcinoma 2 2 4 3 2 Ajay Kumar Chaudhary Shruti Pandya Ravi Mehrotra Alok C Bharti Mangal Singh Mamta Singh Abstract Background Oral malignancy is a major global health problem. Besides the main risk factors of tobacco smoking and alcohol infection by human papillomavirus HPV and genetic alterations are likely to play an important role in these lesions. The purpose of this study was to compare the efficacy of HC-II assay and PCR for the detection of specific HPV type HPV 16 E6 in OSMF and OSCC cases as well as find out the prevalence of the high risk HPV HR-HPV in these lesions. Methods and materials Four hundred and thirty patients of the potentially malignant and malignant oral lesions were taken from the Department of Otorhinolaryngology Moti Lal Nehru Medical College Allahabad India from Sept 2007-March 2010. Of which 208 cases were oral submucous fibrosis OSMF and 222 cases were oral squamous cell carcinoma OSCC . The HC-II assay and PCR were used for the detection of HR-HPV DNA. Result The overall prevalence of HR-HPV 16 E6 DNA positivity was nearly 26 by PCR and by the HC-II assay in case of potentially malignant disorder of the oral lesions such as OSMF. However in case of malignant oral lesions such as OSCC HPV 16 E6 positive by PCR and by the HC-II assay. In case of OSMF the two test gave concordant result for 42 positive samples and 154 negative samples with an overall level of agreement of Cohen s kappa 95 CI . The sensitivity and specificity of the test were and p . In case of OSCC the two test gave concordant result for 61 positive samples and 152 negative samples with an overall level of agreement of .
