Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Characterization of an H3N2 triple reassortant influenza virus with a mutation at the receptor binding domain (D190A) that occurred upon virus transmission from turkeys to pigs | Yassine et al. Virology Journal 2010 7 258 http content 7 1 258 VIROLOGY JOURNAL SHORT REPORT Open Access Characterization of an H3N2 triple reassortant influenza virus with a mutation at the receptor binding domain D190A that occurred upon virus transmission from turkeys to pigs Hadi M Yassine1 2 Mahesh Khatri1 Chang W Lee1 Yehia M Saif1 Abstract The hemagglutinin HA protein of influenza virus mediates essential viral functions including the binding to host receptor and virus entry. It also has the antigenic sites required for virus neutralization by host antibodies. Here we characterized an H3N2 triple reassortant TR influenza virus A turkey Ohio 313053 04 with a mutation at the receptor binding domain Asp190Ala that occurred upon virus transmission from turkeys to pigs in an experimental infection study. The mutant virus replicated less efficiently than the parental virus in human pig and turkey primary tracheal bronchial epithelial cells with more than 3-log10 difference in virus titer at 72 hours post infection. In addition the mutant virus demonstrated lower binding efficiency to plasma membrane preparations from all three cell types compared to the parental virus. Antisera raised against the parental virus reacted equally to both homologous and heterlogous viruses however antisera raised against the mutant virus showed 4-8 folds lower reactivity to the parental virus. Introduction Influenza A viruses infect a wide range of animal species including mammals and birds 1 . All subtypes have been isolated from avian species however few subtypes have circulated and caused disease in mammals 2 . Generally speaking avian viruses preferentially bind to N-acetylneuraminic acid-a2 3-galactose form of sialic acid a2 . receptors while human viruses preferentially bind to a2 . receptors 3 . The HA is a major surface glycoprotein on influenza virus envelope and is essential for binding to host receptors and virus entry 4 . In addition it .
