Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Simultaneous detection and differentiation by multiplex real time RT-PCR of highly pathogenic avian influenza subtype H5N1 classic (clade proper) and escape mutant (clade variant) lineages in Egypt | Abdelwhab et al. Virology Journal 2010 7 260 http content 7 1 260 VIROLOGY JOURNAL METHODOLOGY Open Access Simultaneous detection and differentiation by multiplex real time RT-PCR of highly pathogenic avian influenza subtype H5N1 classic clade proper and escape mutant clade variant lineages in Egypt cl c - r I h z A k I - h A p - k1 2 3 A h z D K 2 K I r f- I K CT KI I K 1 h ỉ ri z 7 ill -1r1A IC- 1-f-- r A 2 K I ỉ r D r r K 1 El-sayed M Abdelwnab AnmeQ M ErTan Christian Grund Mario Ziller Abdel-satar AidTd Martin Deer Mona M Aly2 HaTez M HaTez3 Timm C Harder1 Abstract Background The endemic status oT highly pathogenic avian influenza virus HPAIV oT subtype H5N1 in Egypt continues to devastate the local poultry industry and poses a permanent threat Tor human health. Several genetically and antigenically distinct H5N1 lineages co-circulate in Egypt Strains oT clade proper replicate mainly in backyard birds causing the bulk oT human inTections while a variant lineage within appears to be perpetuated mainly in commercial poultry Tarms in Egypt. Viruses oT the lineage represent driTt variants escaping Trom conventional vaccine-induced immunity and some oT these strains also escaped detection by commercial real time reverse transcriptase PCR RT-qPCR protocols due to mismatches in the primers probe binding sites. Results We developed therefore a versatile sensitive and lineage-speciTic multiplex RT-qPCR Tor detection and typing oTH5N1 viruses in Egypt. Analytical characterization was carried out using 50 Egyptian HPAIV H5N1 strains isolated since 2006 and 45 other avian inTluenza viruses AIV . A detection limit oT400 cRNA copies per ml sample matrix was Tound. Higher diagnostic sensitivity oT the multiplex assay in comparison to other generic H5 or M-gene based RT-qPCR assays were Tound by examination oT 63 swab samples Trom experimentally inTected chickens and 50 AIV-positive swab samples Trom diTTerent host .
