Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Development and evaluation of an immunochromatographic strip test based on the recombinant UL51 protein for detecting antibody against duck enteritis virus | Shen et al. Virology Journal 2010 7 268 http content 7 1 268 VIROLOGY JOURNAL RESEARCH Open Access Development and evaluation of an immunochromatographic strip test based on the recombinant UL51 protein for detecting antibody against duck enteritis virus 1 1 2 T- I- 1 1 Chanjuan Shen Anchun Cheng Mingshu Wang Kunfeng Sun Renyong Jia Tao Sun Na Zhang Dekang Zhu1 2 Qihui Luo2 Yi Zhou2 Xiaoyue Chen1 2 3 Abstract Background Duck enteritis virus DEV infection causes substantial economic losses to the worldwide duckproducing areas. The monitoring of DEV-specific antibodies is a key to evaluate the effect of DEV vaccine and develop rational immunization programs. Thus in this study an immunochromatographic strip ICS test was developed for detecting DEV serum antibodies. Results The ICS test is based on membrane chromatography and uses both the purified recombinant UL51 protein conjugated with colloidal gold and goat anti-rabbit IgG conjugated with colloidal gold as tracers the purified recombinant UL51 protein as the capture reagent at the test line and rabbit IgG as the capture reagent at the control line. The specificity of the ICS was evaluated by sera against DEV Duck hepatitis virus DHV Riemerella anatipestifer RA Duck E. coli Muscovy duck parvovirus MPV or Duck Influenza viruses DIV . Only sera against DEV showed the strong positive results. In order to determine the sensitivity of the ICS anti-DEV serum diluted serially was tested and the minimum detection limit of 1 128 was obtained. The ICS components which are provided in a sealed package require no refrigeration and are stable for 12 months. To evaluate the effect of the ICS 110 duck serum samples collected from several non-immune duck flocks were simultaneously tested by the ICS test enzyme-linked immunosorbent assay ELISA and neutralization test NT . The results showed that the sensitivity of the ICS test was almost consistent with ELISA and much higher than NT has low cost and .
