Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Metagenomic analysis of the turkey gut RNA virus community | Day et al. Virology Journal 2010 7 313 http content 7 1 313 VIROLOGY JOURNAL RESEARCH Open Access Metagenomic analysis of the turkey gut RNA virus community 1 2 2 2 1 J Michael Day Linda L Ballard Mary V Duke Brian E Scheffler Laszlo Zsak Abstract Viral enteric disease is an ongoing economic burden to poultry producers worldwide and despite considerable research no single virus has emerged as a likely causative agent and target for prevention and control efforts. Historically electron microscopy has been used to identify suspect viruses with many small round viruses eluding classification based solely on morphology. National and regional surveys using molecular diagnostics have revealed that suspect viruses continuously circulate in United States poultry with many viruses appearing concomitantly and in healthy birds. High-throughput nucleic acid pyrosequencing is a powerful diagnostic technology capable of determining the full genomic repertoire present in a complex environmental sample. We utilized the Roche 454 Life Sciences GS-FLX platform to compile an RNA virus metagenome from turkey flocks experiencing enteric disease. This approach yielded numerous sequences homologous to viruses in the BLAST nr protein database many of which have not been described in turkeys. Our analysis of this turkey gut RNA metagenome focuses in particular on the turkey-origin members of the Picornavirales the Caliciviridae and the turkey Picobirnaviruses. Introduction Enteric disease syndromes such as Poult Enteritis Complex PEC in young turkeys and Runting-Stunting Syndrome RSS in chickens are a continual economic burden for poultry producers. The only reliable method to reproduce the clinical signs of these syndromes in experimental birds is oral inoculation with crude preparations of intestinal contents from naturally infected birds. Further the full spectrum of the field signs observed associated with these syndromes is difficult to reproduce experimentally with
