Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: “pp65 antigenemia and real time polymerase chain reaction (PCR) based-study to determine the prevalence of human cytomegalovirus (HCMV) in kidney donors and recipients with follow-up studies.” | Madhavan et al. Virology Journal 2010 7 322 http content 7 1 322 VIROLOGY JOURNAL RESEARCH Open Access pp65 antigenemia and real time polymerase chain reaction PCR based-study to determine the prevalence of human cytomegalovirus HCMV in kidney donors and recipients with follow-up studies. 1 11 2 1 Hajib N Madhavan Moses Y Samson Murali Ishwarya Ramanathan Vijayakumar Malathi Jambulingam Abstract Background The present study was undertaken to determine the rate of occurrence of Human cytomegalovirus HCMV among kidney transplant recipients and donors by application of direct detection methods and to understand HCMV infection disease development among transplanted patients as a prospective study. Results Peripheral blood samples collected from 76 kidney donors and 76 recipients from September 2007 to August 2009 were subjected to pp65 antigenemia and Quantitative real-time PCR qRT-PCR assays. Data were analyzed under Group A B and C. Group A was further divided into sub-groups I II III IV and V for better understanding. Three one and two donors in sub-group I III IV of Group A tested positive for real time PCR respectively. One recipient from group III tested positive for HCMV by qRT- PCR prior transplantation and remained positive one month post-transplantation. Three other recipients tested negative prior to transplantation became positive a month after transplantation. Group B consisted of 18 donor-recipient pairs and one of the donor tested positive for HCMV by qRT-PCR. Eight recipients tested positive for HCMV one month after transplantation. The pp65 positivity and HCMV DNA load was high among group C recipients who mostly had symptoms of active disease. Significantly high values of pp65 antigenemia were observed among recipients of sub-group II non-parametric chi-square test p . Positive correlation between pp65 antigenemia and qRT-PCR value was observed. Thirty three of the recipients with disease treated with Valgancyclovir showed .
