Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Hepatitis C virus, human immunodeficiency virus and pseudomonas phage PS5 triad share epitopes of immunogenic determinants | Golkar and Jamil Virology Journal 2010 7 346 http content 7 1 346 VIROLOGY JOURNAL RESEARCH Open Access Hepatitis C virus human immunodeficiency virus and pseudomonas phage PS5 triad share epitopes of immunogenic determinants Zhabiz Golkar Nusrat Jamil Abstract A lytic phage for Pseudomonas aeruginosa belongs to the Myoviridea family was isolated from urine for use in therapeutics. Pair of hepatitis C virus HCV primers highlighted segments on the genome of this phage. The sequence of these PCR products as well as the possible serological cross reactivity relationship between HCV and the phage were investigated. One hundred HCV positive human sera were analyzed by ELISA. Ninety six well plates were coated with multiple epitopes of HCV proteins Kit phage and Pseudomonas cells. Initially the positive and negative control sera supplied in the test kit were used to evaluate the cross reactivity between the phage and anti-HCV antibodies. The results suggested a value over than for a HCV positive reaction. Of the 100 HCV positive sera tested sixty five and thirty percent showed cross reaction with phage lysate and Pseudomonas aeruginosa respectively. High HCV antibody titer correlated to high cut off value for phage cross reaction whereas no such correlation existed between HCV antibody titer and Pseudomonas cross reaction. The PCR products were sequenced and aligned with the HCV genome of H77. Sequence homology was detected in the 5 3 UTRs and NS3 regions. Further these products showed similarity with HIV-1 Env Pol 3 LTR regions as well. Introduction Hepatitis C virus HCV currently infects an estimated 3 of people worldwide encodes several proteins. HCV displays numerous interactions with the immune systems. Consequently a number of auto-antibodies are observed during the course of hepatitis C 1 . Many studies have detected the presence of antibodies reactive to a cloned host derived auto-antigen GOR and are highly correlated with the presence of
