Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification and expression analysis of WRKY transcription factor genes in canola (Brassica napus L.) in response to fungal pathogens and hormone treatments | BMC Plant Biology BioMed Central Open Access Research article Identification and expression analysis of WRKY transcription factor genes in canola Brassica napus L. in response to fungal pathogens and hormone treatments Bo Yang1 Yuanqing Jiang2 Muhammad H Rahman1 Michael K Deyholos2 and Nat NV Kav 1 Address Department of Agricultural Food and Nutritional Science Edmonton Alberta T6G 2P5 Canada and 2Department of Biological Sciences University of Alberta Edmonton Alberta T6G 2E9 Canada Email Bo Yang - byang@ Yuanqing Jiang - yuanqing@ Muhammad H Rahman - mrahman@ Michael K Deyholos - deyholos@ Nat NV Kav - nat@ Corresponding author Published 3 June 2009 Received 30 October 2008 BMC Plant Biology 2009 9 68 doi 1471-2229-9-68 Accepted 3 June 2009 This article is available from http 1471-2229 9 68 2009 Yang et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Members of plant WRKY transcription factor families are widely implicated in defense responses and various other physiological processes. For canola Brassica napus L. no WRKY genes have been described in detail. Because of the economic importance of this crop and its evolutionary relationship to Arabidopsis thaliana we sought to characterize a subset of canola WRKYgenes in the context of pathogen and hormone responses. Results In this study we identified 46 WRKY genes from canola by mining the expressed sequence tag EST database and cloned cDNA sequences of 38 BnWRKYs. A phylogenetic tree was constructed using the conserved WRKY domain amino acid sequences which demonstrated that BnWRKYs can be divided into three major groups. We further compared BnWRKYs to the 72 WRKY
