Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Selection of reference genes for quantitative real-time PCR expression studies in the apomictic and sexual grass Brachiaria brizantha | BMC Plant Biology BioMed Central Open Access Selection of reference genes for quantitative real-time PCR expression studies in the apomictic and sexual grass Brachiaria brizantha Érica Duarte Silveira 1 2 Márcio Alves-Ferreira2 Larissa Arrais Guimarães1 Felipe Rodrigues da Silva1 and Vera Tavares de Campos Carneiro 1 Address 1Embrapa Genetic Resources and Biotechnology Parque Estaẹão Biológica PqEB Av. W5 Norte final Caixa Postal 02372 Brasilia Brasil and 2Department of Genetics Federal University of Rio de Janeiro Av. Prof. Rodolpho Paulo Rocco s n Prédio do CCS Instituto de Biologia 2o Andar - Rio de Janeiro RJ Brasil Email Érica Duarte Silveira - ericads@ Márcio Alves-Ferreira - alvesfer@ Larissa Arrais Guimarães - larissaarrais@ Felipe Rodrigues da Silva - felipes@ Vera Tavares de Campos Carneiro - vera@ Corresponding author Published 2 July 2009 Received 25 November 2008 BMC Plant Biology 2009 9 84 doi 1471-2229-9-84 Accepted 2 July 2009 This article is available from http 1471-2229 9 84 2009 Silveira et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Brachiaria brizantha is an important forage grass. The occurrence of both apomictic and sexual reproduction within Brachiaria makes it an interesting system for understanding the molecular pathways involved in both modes of reproduction. Quantitative real time PCR qRT-PCR has emerged as an important technique to compare expression profile of target genes and in order to obtain reliable results it is important to have suitable reference genes. In this work we evaluated eight potential reference genes for B. brizantha qRT-PCR experiments .
