Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Analysis of a post-translational steroid induction system for GIGANTEA in Arabidopsis | BMC Plant Biology BioMed Central Research article Analysis of a post-translational steroid induction system for GIGANTEA in Arabidopsis Markus Gunl Eric FungMin Liew Karine David and Joanna Putterill Address Plant Molecular Sciences School of Biological Sciences University of Auckland Private Bag 92019 Auckland New Zealand Email Markus Gunl - gunlm@ Eric FungMin Liew-mac_ming@ Karine David - Joanna Putterill - Corresponding author Open Access Published 30 November 2009 BMC Plant Biology 2009 9 141 doi l47l-2229-9-l4l Received 30 June 2009 Accepted 30 November 2009 This article is available from http l47l-2229 9 l4l 2009 Gunl et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background To investigate the link between the flowering time gene GIGANTEA GI and downstream genes an inducible GI system was developed in Arabidopsis thaliana L. Heynh. Transgenic Arabidopsis plant lines were generated with a steroid-inducible post-translational control system for GI. The gene expression construct consisted of the coding region of the GI protein fused to that of the ligand binding domain of the rat glucocorticoid receptor GR . This fusion gene was expressed from the constitutive cauliflower mosaic virus 35S promoter and was introduced into plants carrying the gi-2 mutation. Application of the steroid dexamethasone DEX was expected to result in activation of the GI-GR protein and its relocation from the cytoplasm to the nucleus. Results Application of DEX to the transgenic plant lines rescued the late flowering phenotype conferred by the gi-2
