Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A high-density collection of EMS-induced mutations for TILLING in Landsberg erecta genetic background of Arabidopsis | BMC Plant Biology BioMed Central Open Access Methodology article A high-density collection of EMS-induced mutations for TILLING in Landsberg erecta genetic background of Arabidopsis Beatriz Martín Mercedes Ramiro José M Martinez-Zapater and Carlos Alonso-Blanco Address Departamento de Genética Molecular de Plantas Centro Nacional de Biotecnología CNB Consejo Superior de Investigaciones Científicas CSIC Madrid-28049 Spain Email Beatriz Martín - bmartin@ Mercedes Ramiro - mramiro@ José M Martínez-Zapater - zapater@ Carlos Alonso-Blanco - calonso@ Corresponding author Published 14 December 2009 BMC Plant Biology 2009 9 147 doi l47l-2229-9-l47 Received 6 July 2009 Accepted l4 December 2009 This article is available from http l47l-2229 9 l47 2009 Martín et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Arabidopsis thaliana is the main model species for plant molecular genetics studies and world-wide efforts are devoted to identify the function of all its genes. To this end reverse genetics by TILLING Targeting Induced Local Lesions IN Genomes in a permanent collection of chemically induced mutants is providing a unique resource in Columbia genetic background. In this work we aim to extend TILLING resources available in A. thaliana by developing a new population of ethyl methanesulphonate EMS induced mutants in the second commonest reference strain. In addition we pursue to saturate the number of EMS induced mutations that can be tolerated by viable and fertile plants. Results By mutagenizing with different EMS concentrations we have developed a permanent collection of 37l2 M2 M3 independent mutant lines in the reference strain .
