Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Deep sequencing identifies novel and conserved microRNAs in peanuts (Arachis hypogaea L.) | Zhao et al. BMC Plant Biology 2010 10 3 http 1471-2229 10 3 BMC Plant Biology RESEARCH ARTICLE Open Access Deep sequencing identifies novel and conserved microRNAs in peanuts Arachis hypogaea L. z I 71 71 1 74 1 1 1 4 -T- I rx I R X X 4 s X rx 1 rx 1 A I 1 9 A A 1 1 17 Chuan-Zhi Zhao Han Xia 2 Taylor Price Frazier3 Ying-Yin Yao Yu-Ping K Ai-Qin II Meng-Jun I Chang-Sheng Li1 2 Bao-Hong Zhang2 Xing-Jun Wang1 2 Abstract Background MicroRNAs miRNAs are a new class of small endogenous RNAs that play a regulatory role in the cell by negatively affecting gene expression at the post-transcriptional level. miRNAs have been shown to control numerous genes involved in various biological and metabolic processes. There have been extensive studies on discovering miRNAs and analyzing their functions in model species such as Arabidopsis and rice. Increasing investigations have been performed on important agricultural crops including soybean conifers and Phaselous vulgaris but no studies have been reported on discovering peanut miRNAs using a cloning strategy. Results In this study we employed the next generation high through-put Solexa sequencing technology to clone and identify both conserved and species-specific miRNAs in peanuts. Next generation high through-put Solexa sequencing showed that peanuts have a complex small RNA population and the length of small RNAs varied 24nt being the predominant length for a majority of the small RNAs. Combining the deep sequencing and bioinformatics we discovered 14 novel miRNA families as well as 75 conserved miRNAs in peanuts. All 14 novel peanut miRNAs are considered to be species-specific because no homologs have been found in other plant species except ahy-miRn1 which has a homolog in soybean. qRT-PCR analysis demonstrated that both conserved and peanut-specific miRNAs are expressed in peanuts. Conclusions This study led to the discovery of 14 novel and 22 conserved miRNA families from peanut. These results show .
