Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Genetic relationships among seven sections of genus Arachis studied by using SSR markers | Koppolu et al. BMC Plant Biology 2010 10 15 http 1471-2229 10 15 BMC Plant Biology RESEARCH ARTICLE Open Access Genetic relationships among seven sections of genus Arachis studied by using SSR markers Ravi Koppolu1 Hari D Upadhyaya1 Sangam L Dwivedi1 David A Hoisington1 Rajeev K Varshney1 2 Abstract Background The genus Arachis originated in South America is divided into nine taxonomical sections comprising of 80 species. Most of the Arachis species are diploids 2n 2x 20 and the tetraploid species 2n 2x 40 are found in sections Arachis Extranervosae and Rhizomatosae. Diploid species have great potential to be used as resistance sources for agronomic traits like pests and diseases drought related traits and different life cycle spans. Understanding of genetic relationships among wild species and between wild and cultivated species will be useful for enhanced utilization of wild species in improving cultivated germplasm. The present study was undertaken to evaluate genetic relationships among species 96 accessions belonging to seven sections of Arachis by using simple sequence repeat SSR markers developed from Arachis hypogaea genomic library and gene sequences from related genera of Arachis. Results The average transferability rate of 101 SSR markers tested to section Arachis and six other sections was 81 and 59 respectively. Five markers IPAHM 164 IPAHM 165 IPAHM 407a IPAHM 409 and IPAHM 659 showed 100 transferability. Cluster analysis of allelic data from a subset of 32 SSR markers on 85 wild and 11 cultivated accessions grouped accessions according to their genome composition sections and species to which they belong. A total of 109 species specific alleles were detected in different wild species Arachis pusilla exhibited largest number of species specific alleles 15 . Based on genetic distance analysis the A-genome accession ICG 8200 A. duranensis and the B-genome accession ICG 8206 A. ipaensis were found most closely related to A. .
