Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification and functional characterisation of the promoter of the calcium sensor gene CBL1 from the xerophyte Ammopiptanthus mongolicus | Guo et al. BMC Plant Biology 2010 10 18 http 1471-2229 10 18 BMC Plant Biology RESEARCH ARTICLE Open Access Identification and functional characterisation of the promoter of the calcium sensor gene CBL1 from the xerophyte Ammopiptanthus mongolicus Lili Guo Yanhua Yu Xinli Xia Weilun Yin Abstract Background CBL1 is a calcium sensor that regulates drought cold and salt signals in Arabidopsis. Overexpression of CBL1 gene in Arabidopsis and in Ammopiptanthus mongolicus showed different tolerant activities. We are interested in understanding the molecular mechanism of the upstream region of the CBL1 gene of A. mongolicus AmCBLl . We investigated and characterized the promoter of the AmCBLl gene for promoters play a very important role in regulating gene expression in eukaryotes. Results A 1683-bp 5 flanking region was isolated from A. mongolicus. The sequence was identified as AmCBLl promoter. Analysis of the promoter sequence indicated a 690-bp intron and some basic cis-acting elements were related to various environmental stresses and plant hormones. To identify the functional region of the AmCBLl promoter five plant expression vectors fused with the GUS b-glucuronidase gene driven by series deleted fragments of AmCBLl promoter at different lengths from -1659 -1414 -1048 -296 to -167 bp relative to the transcriptional start site were constructed and transformed into Nicotiana tabacum L. cv. 89. Functional properties of each promoter segment were examined by GUS staining and fluorescence quantitative analyses using at least three single-copy PCR-positive plants of transgenic tobacco treated with various environmental stresses and plant hormones for different times. We demonstrated that the AmCBL1 promoter was a vascular-specific and multiplestress-inducible promoter. Our results further imply that the promoter fragment B1S3 possessed sufficient essential cis-acting elements accounting for vascular-specific and stress-induced expression patterns. .
