Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Iron and ferritin accumulate in separate cellular locations in Phaseolus seeds | Cvitanich et al. BMC Plant Biology 2010 10 26 http 1471-2229 10 26 BMC Plant Biology RESEARCH ARTICLE Open Access Iron and ferritin accumulate in separate cellular locations in Phaseolus seeds Cristina Cvitanich1 Wojciech J Przybytowicz2 3 Dorian F Urbanski1 Anna M Jurkiewicz1 Jolanta Mesjasz-Przybytowicz2 Matthew W Blair4 Carolina Astudillo4 Erik 0 Jensen1 Jens Stougaard1 Abstract Background Iron is an important micronutrient for all living organisms. Almost 25 of the world population is affected by iron deficiency a leading cause of anemia. In plants iron deficiency leads to chlorosis and reduced yield. Both animals and plants may suffer from iron deficiency when their diet or environment lacks bioavailable iron. A sustainable way to reduce iron malnutrition in humans is to develop staple crops with increased content of bioavailable iron. Knowledge of where and how iron accumulates in seeds of crop plants will increase the understanding of plant iron metabolism and will assist in the production of staples with increased bioavailable iron. Results Here we reveal the distribution of iron in seeds of three Phaseolus species including thirteen genotypes of P. vulgaris P. coccineus and P. lunatus. We showed that high concentrations of iron accumulate in cells surrounding the provascular tissue of P. vulgaris and P. coccineus seeds. Using the Perls Prussian blue method we were able to detect iron in the cytoplasm of epidermal cells cells near the epidermis and cells surrounding the provascular tissue. In contrast the protein ferritin that has been suggested as the major iron storage protein in legumes was only detected in the amyloplasts of the seed embryo. Using the non-destructive micro-PIXE Particle Induced X-ray Emission technique we show that the tissue in the proximity of the provascular bundles holds up to 500 pg g-1 of iron depending on the genotype. In contrast to P. vulgaris and P. coccineus we did not observe iron accumulation in the .
