Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Genetic transformation of cotton with a harpin-encoding gene hpaXoo confers an enhanced defense response against different pathogens through a priming mechanism | Miao et al. BMC Plant Biology 2010 10 67 http 1471-2229 10 67 BMC Plant Biology RESEARCH ARTICLE _ Open Access Genetic transformation of cotton with a harpin-encoding gene hpaXoo confers an enhanced defense response against different pathogens through a priming mechanism Weiguo Miao 1 2 Xiben Wang23 Ming Li 1 Congfeng Song 1 Yu Wang1 Dongwei Hu4 and Jinsheng Wang 1 Abstract Background The soil-borne fungal pathogen Verticillium dahliae Kleb causes Verticillium wilt in a wide range of crops including cotton Gossypium hirsutum . To date most upland cotton varieties are susceptible to V dahliae and the breeding for cotton varieties with the resistance to Verticillium wilt has not been successful. Results Hpa1Xoo is a harpin protein from Xanthomonas oryzae pv. oryzae which induces the hypersensitive cell death in plants. When hpa1Xoo was transformed into the susceptible cotton line Z35 through Agrobacterium-mediated transformation the transgenic cotton line T-34 with an improved resistance to Verticillium dahliae was obtained. Cells of the transgenic T-34 when mixed with the conidia suspension of V dahliae had a higher tolerance to V dahliae compared to cells of untransformed Z35. Cells of T-34 were more viable 12 h after mixing with V dahliae conidia suspension. Immunocytological analysis showed that Hpa1Xoo expressed in T-34 accumulated as clustered particles along the cell walls of T-34. In response to the infection caused by V dahliae the microscopic cell death and the generation of reactive oxygen intermediates were observed in leaves of T-34 and these responses were absent in leaves of Z35 inoculated with V dahliae. Quantitative RT-PCR analysis indicated that five defense-related genes ghAOXl hinl nprl ghdhg-OMT and hsr203J were up-regulated in T-34 inoculated with V dahliae. The up-regulations of these defense-relate genes were not observed or in a less extent in leaves of Z-35 after the inoculation. Conclusions Hpa1Xoo accumulates along .
