Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Efficient inhibition of hepatitis B virus replication by hepatitis delta virus ribozymes delivered by targeting retrovirus | Wang et al. Virology Journal 2010 7 61 http content 7 1 61 VIROLOGY JOURNAL RESEARCH Open Access Efficient inhibition of hepatitis B virus replication by hepatitis delta virus ribozymes delivered by targeting retrovirus Chuan-Xi Wang 1 2 Yan-Qin Lu2 Peng Qi2 Long-Hua Chen 1 and Jin-Xiang Han 2 Abstract Background Hepatitis delta virus HDV ribozyme is an attractive molecular tool that can specifically recognize and catalyze the self-cleavage of the viral RNA phosphodiester backbone. However a major obstacle in the medical application of the HDV ribozyme is the lack of specificity in the delivery of the ribozyme to defined target cells. Results The objective of this study was to determine whether retroviral vectors can deliver the HDV ribozyme into the target cells and to elucidate whether HDV ribozyme plays a role in hepatitis B virus HBV replication. In our study the transduction of helper-free pseudotyped retrovirus which showed a broad host range in human hepatoma cells was performed under 2 conditions that is in the presence of polymerized human serum albumin pHSA and in the absence of pHSA. The transduction ability in the presence of pHSA was higher than in the absence of pHSA. Moreover HBsAg and HBeAg levels after transductions with pHSA were significantly lower than those in the absence of pHSA thus indicating that the recombinant retrovirus had HBV-specific cleavage activity and targeted HepG2215 cells. Conclusions These data suggest that this system provides a new approach for targeting hepatocytes and has a great potential in gene therapy for HBV infection. Introduction Hepatitis B virus HBV causes acute and chronic infections of the liver. Acute infections can cause serious illnesses and lead to fatal fulminant hepatitis in approximately of the patients. Chronic infections may also induce serious consequences leading to untreat-able hepatocellular carcinoma HCC in nearly 25 of the patients. The number of deaths attributed to .
