Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Keratin 18 phosphorylation as a progression marker of chronic hepatitis B | Shi et al. Virology Journal 2010 7 70 http content 7 1 70 VIROLOGY JOURNAL RESEARCH Open Access Keratin 18 phosphorylation as a progression marker of chronic hepatitis B 1 2 1 T- 71 2 2 w Z-I 2 2 X 71 1 Ying Shi Shihui Sun Yali Liu Junfeng Li Tong Zhang Hao Wu Xinyue Chen Dexi Chen Yusen Zhou Abstract Background The intermediate filament proteins keratins 18 K18 and 8 K8 polymerize to form the cytoskeletal network in the mature hepatocytes. It has been shown that the phosphorylation of K18 at two serine residues 33 and 52 correlates with the progression of hepatitis C but little is known of chronic hepatitis B CHB . In this study we examined K18 phosphorylation in relation to CHB. Results Site-specific phosphorylation of K18 was determined in livers of twelve healthy donors and non-cirrhosis n 40 and cirrhosis n 21 patients. On average progressively higher level of Ser52 phosphorylation was observed in non-cirrhotic and cirrhotic livers while elevated Ser33 phosphorylation was detected in both livers but no significant difference. Progressive increase of Ser33 and Ser52 phosphorylation correlated with the elevation of both histological lesions and enzymatic activities of alanine aminotransferase in non-cirrhotic livers. In the hepatocytes of an inactive HBV carrier strong signals of Ser33 phosphorylation were co-localized with viral infection while only basal level of Ser52 phosphorylation was detected in infected cells. Conclusion Assuming all obtained data our data suggest that K18 phosphorylation is a progression marker for CHB. Background Keratin 18 K18 is a member of the intermediate filament family comprising 70 cytoskeleton proteins. Adult hepatocytes contain only K18 and keratin 8 K8 heteropolymerized to form the filament networks that protect the cells from various mechanical stresses 1-3 . Serious hepatocellular injures usually result in damages in the filament scaffolds. For example during apoptosis K18 is cleaved into small .
