Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Interactions of SARS Coronavirus Nucleocapsid Protein with the host cell proteasome subunit p4 | Wang et al. Virology Journal 2010 7 99 http content 7 1 99 VIROLOGY JOURNAL RESEARCH Open Access Interactions of SARS Coronavirus Nucleocapsid Protein with the host cell proteasome subunit p42 Qin Wang 1 Chuan Li2 Quanfu Zhang1 Tao Wang2 Jiandong Li1 Wuxiang Guan1 Jianshi Yu1 Mifang Liang 2 and Dexin Li 1 Abstract Background Severe acute respiratory syndrome-associated coronavirus SARS-CoV spreads rapidly and has a high case-mortality rate. The nucleocapsid protein NP of SARS-CoV may be critical for pathogenicity. This study sought to discover the host proteins that interact with SARS-CoV NP Results Using surface plasmon resonance biomolecular interaction analysis SPR BIA and matrix-assisted laser desorption ionization time of flight MALDI-TOF mass spectrometry we found that only the proteasome subunit p42 from human fetal lung diploid fibroblast 2BS cells bound to SARS-CoV NP. This interaction was confirmed by the glutathione S-transferase GST fusion protein pulldown technique. The co-localization signal of SARS-CoV NP and proteasome subunit p42 in 2BS cells was detected using indirect immunofluorescence and confocal microscopy. p42 is a subunit of the 26S proteasome this large multi-protein complex is a component of the ubiquitin-proteasome pathway which is involved in a variety of basic cellular processes and inflammatory responses. Conclusion To our knowledge this is the first report that SARS-CoV NP interacts with the proteasome subunit p42 within host cells. These data enhance our understanding of the molecular mechanisms of SARS-CoV pathogenicity and the means by which SARS-CoV interacts with host cells. Background The outbreak of severe acute respiratory syndrome SARS which began in the Guangdong Province of China spread rapidly to more than 30 countries during 2003. SARS has an acute onset is highly transmissible and has a high case-mortality rate approximately 10 1 2 . During SARS infection three phases of viral replication result in .
