Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: The novel HSV-1 US5-1 RNA is transcribed off a domain encoding US 5, US 4, US 3, US 2 and α22 | Jovasevic and Roizman Virology Journal 2010 7 103 http content 7 1 103 VIROLOGY JOURNAL SHORT REPORT Open Access The novel HSV-1 US5-1 RNA is transcribed off a domain encoding US 5 US 4 US 3 US 2 and a22 Vladimir Jovasevic and Bernard Roizman Abstract Background The genome of herpes simplex virus 1 encodes at least 84 transcripts from which proteins are translated and several additional RNAs whose status as mRNAs is unknown. These RNAs include latency-associated transcript OriS1 and OriS2 RNAs and in case of a4 null mutant additional transcript that spans the junction between L and S component of the HSV-1 genome. Current data do not suggest that a peptide is translated from these RNAs. Results We describe here a novel RNA designated US5-1 that spans kb of the unique-short US region. The RNA initiates in US5 and terminates in the a22 open reading frame. It is expressed antisense to US5 US4 US3 and ICP22 mRNAs. This transcript is expressed with Y2 kinetics and has a half-life of 80 minutes. Conclusion These results identify a novel transcript encoded within HSV-1 genome. Since no major hypothetical open-reading frames are present in this transcript it is feasible that this RNA exerts its function as a non-coding RNA. Background The original report of the organization of the unique short US DNA sequences of the herpes simplex virus 1 HSV-1 listed 12 open reading frames ORFs designated US1 a22 through US12 a47 1 . Subsequent studies led to the discovery of three additional transcripts and at least two non coding transcripts. The coding transcripts were mRNA co-terminal with US8 and US9 2 co-terminal with the a22 transcript 3 and co-terminal with the US3 mRNA 4 . The non coding transcript OriS1 initiated in a22 or a47 ORFs run across the origins of DNA synthesis and terminated at the transcription termination site of the a4 gene. OriS2 terminated at or near the transcription initiation site of the a22 or a47 gene 5 . .
