Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A highly attenuated recombinant human respiratory syncytial virus lacking the G protein induces long-lasting protection in cotton rats | Widjojoatmodjo et al. Virology Journal 2010 7 114 http content 7 1 114 VIROLOGY JOURNAL RESEARCH Open Access A highly attenuated recombinant human respiratory syncytial virus lacking the G protein induces long-lasting protection in cotton rats Myra N Widjojoatmodjo 1 Jolande Boes1 Marleen van Bers1 Yvonne van Remmerden1 Paul JM Roholl2 and Willem Luytjes1 Abstract Background Respiratory syncytial virus RSV is a primary cause of serious lower respiratory tract illness for which there is still no safe and effective vaccine available. Using reverse genetics recombinant r RSV and an rRSV lacking the G gene AG were constructed based on a clinical RSV isolate strain 98-25147-X . Results Growth of both recombinant viruses was equivalent to that of wild type virus in Vero cells but was reduced in human epithelial cells like Hep-2. Replication in cotton rat lungs could not be detected for AG while rRSV was 100-fold attenuated compared to wild type virus. Upon single dose intranasal administration in cotton rats both recombinant viruses developed high levels of neutralizing antibodies and conferred comparable long-lasting protection against RSV challenge protection against replication in the lungs lasted at least 147 days and protection against pulmonary inflammation lasted at least 75 days. Conclusion Collectively the data indicate that a single dose immunization with the highly attenuated AG as well as the attenuated rRSV conferred long term protection in the cotton rat against subsequent RSV challenge without inducing vaccine enhanced pathology. Since AG is not likely to revert to a less attenuated phenotype we plan to evaluate this deletion mutant further and to investigate its potential as a vaccine candidate against RSV infection. Background Respiratory syncytial virus RSV is a non-segmented negative-stranded RNA virus and a member of the Paramyxoviridae family. RSV is the most important cause of serious respiratory tract disease for young infants and
