Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Concentration of acrylamide in a polyacrylamide gel affects VP4 gene coding assignment of group A equine rotavirus strains with P[12] specificity | Long-Croal et al. Virology Journal 2010 7 136 http content 7 1 136 VIROLOGY JOURNAL RESEARCH Open Access Concentration of acrylamide in a polyacrylamide gel affects VP4 gene coding assignment of group A equine rotavirus strains with P 12 specificity LaShanda M Long-Croal21 2 Xiaobo Wen21 Eileen N Ostlund23 and Yasutaka Hoshino 1 Abstract Background It is universally acknowledged that genome segment 4 of group A rotavirus the major etiologic agent of severe diarrhea in infants and neonatal farm animals encodes outer capsid neutralization and protective antigen VP4. Results To determine which genome segment of three group A equine rotavirus strains H-2 FI-14 and FI-23 with P 12 specificity encodes the VP4 we analyzed dsRNAs of strains H-2 FI-14 and FI-23 as well as their reassortants by polyacrylamide gel electrophoresis PAGE at varying concentrations of acrylamide. The relative position of the VP4 gene of the three equine P 12 strains varied either genome segment 3 or 4 depending upon the concentration of acrylamide. The VP4 gene bearing P 3 P 4 P 6 P 7 P 8 or P 18 specificity did not exhibit this phenomenon when the PAGE running conditions were varied. Conclusions The concentration of acrylamide in a PAGE gel affected VP4 gene coding assignment of equine rotavirus strains bearing P 12 specificity. Background Diarrheal disease is one of the principal causes of morbidity and mortality among young children in the developing world. Infectious diarrhea of neonatal animals is also one of the most common and economically devastating conditions encountered in the animal agriculture industry. Among an array of infectious agents including bacteria viruses and parasites group A rotaviruses are the single most important etiologic agents of diarrhea in infants and young children worldwide and in addition they are the most commonly identified viral cause of diarrhea in neonatal food animals 1-4 . In 1975 rotaviruses were first demonstrated being involved in .
