Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A potentially novel overlapping gene in the genomes of Israeli acute paralysis virus and its relatives | Virology Journal BioMed Central Research A potentially novel overlapping gene in the genomes of Israeli acute paralysis virus and its relatives Niv Sabath Nicholas Price and Dan Graur Open Access Address Department of Biology and Biochemistry University of Houston Houston TX 77204 USA Email Niv Sabath - nsabath@ Nicholas Price - price4890@ Dan Graur - dgraur@ Corresponding author Published 17 September 2009 Received 2 July 2009 Accepted 17 September 2009 Virology journal 2009 6 144 doi 1743-422X-6-144 This article is available from http content 6 1 144 2009 Sabath et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The Israeli acute paralysis virus IAPV is a honeybee-infecting virus that was found to be associated with colony collapse disorder. The IAPV genome contains two genes encoding a structural and a nonstructural polyprotein. We applied a recently developed method for the estimation of selection in overlapping genes to detect purifying selection and hence functionality. We provide evolutionary evidence for the existence of a functional overlapping gene which is translated in the 1 reading frame of the structural polyprotein gene. Conserved orthologs of this putative gene which we provisionally call pog predicted overlapping gene were also found in the genomes of a monophyletic clade of dicistroviruses that includes IAPV acute bee paralysis virus Kashmir bee virus and Solenopsis invicta red imported fire ant virus 1. Background Colony collapse disorder CCD is a syndrome characterized by the mass disappearance of honeybees from hives 1 . CCD imperils a global resource estimated at approximately 200 billion 2 . For example it has been estimated that up to 35 of .
