Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A geminiviral amplicon (VA) derived from Tomato leaf curl virus (ToLCV) can replicate in a wide variety of plant species and also acts as a VIGS vector | Virology Journal BioMed Central Research A geminiviral amplicon VA derived from Tomato leaf curl virus ToLCV can replicate in a wide variety of plant species and also acts as a VIGS vector Prerna Pandey Nirupam R Choudhury and Sunil K Mukherjee Open Access Address Plant Molecular Biology Group International Centre for Genetic Engineering and Biotechnology ICGEB Aruna Asaf Ali Marg New Delhi - 110 067 India Email Prerna Pandey - prernapandey@ Nirupam R Choudhury - nirupam@ Sunil K Mukherjee - sunilm@ Corresponding author Published 29 September 2009 Received 31 August 2009 Accepted 29 September 2009 Virologyjournal 2009 6 152 doi l743-422X-6-l52 This article is available from http content 6 l l52 2009 Pandey et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The Tomato leaf curl virus ToLCV belongs to the genus begomoviridae of the family Geminiviridae. The kb DNA genome of the virus encodes all the information required for viral DNA replication transcription and transmission across the plant cells. However all of the genome sequences are not required for viral DNA replication. We attempted to reveal the minimal essential region required for DNA replication and stable maintenance. The phenomenon of Virus Induced Gene Silencing VIGS has recently been observed with several geminiviruses. We investigated whether the minimal replicating region was also capable of producing siRNAs in planta and a VIGS vector could be constructed using the same minimal sequences. Results We have constructed vectors containing various truncated portions of the Tomato leaf curl virus ToLCV genome and established that a segment spanning from common region CR to AC3 .
