Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Stable replication of the EBNA1/OriP-mediated baculovirus vector and its application to anti-HCV gene therapy | Virology Journal BioMed Central Research Stable replication of the EBNAl OriP-mediated baculovirus vector and its application to anti-HCV gene therapy Hitoshi Suzuki1 Norihiko Matsumoto1 Tomoyuki Suzuki1 Myint OO Chang1 and Hiroshi Takaku 1 2 Open Access Address Department of Life and Environmental Sciences Chiba Institute of Technology 2-17-1 Tsudanuma Narashino Chiba 275-0016 Japan and 2High Technology Research Center Chiba Institute of Technology 2-17-1 Tsudanuma Narashino Chiba 275-0016 Japan Email Hitoshi Suzuki - g0473021FE@ Norihiko Matsumoto - Tomoyuki Suzuki - s026079RN@ Myint OO Chang - c_myintoo@ Hiroshi Takaku - Corresponding author Published 2 October 2009 Received 24 June 2009 Accepted 2 October 2009 Virologyjournal 2009 6 156 doi 1743-422X-6-156 This article is available from http content 6 1 156 2009 Suzuki et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Hepatitis C virus HCV is one of the main causes of liver-related morbidity and mortality. Although combined interferon-a-ribavirin therapy is effective for about 50 of the patients with HCV better therapies are needed and preventative vaccines have yet to be developed. Short-hairpin RNAs shRNAs inhibit gene expression by RNA interference. The application of transient shRNA expression is limited however due to the inability of the shRNA to replicate in mammalian cells and its inefficient transduction. The duration of transgene shRNA expression in mammalian cells can be significantly extended using baculovirus-based shRNA-expressing vectors that contain the latent viral protein Epstein-Barr nuclear antigen I
