Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Detection of NP, N3 and N7 antibodies to avian influenza virus by indirect ELISA using yeast-expressed antigens | Virology Journal BioMed Central Research Detection of NP N3 and N7 antibodies to avian influenza virus by indirect ELISA using yeast-expressed antigens Chitra Upadhyay1 Arun Ammayappan1 2 and Vikram N Vakharia 1 Address 1Center of Marine Biotechnology University of Maryland Biotechnology Institute 701 East Pratt Street Baltimore Maryland 212023101 USA and 2Department of Veterinary Medicine University of Maryland College Park Maryland 20742 USA Email Chitra Upadhyay - upadhyay@ Arun Ammayappan - ammayapp@ Vikram N Vakharia - vakharia@ Corresponding author Open Access Published 7 October 2009 Received 28 June 2009 Accepted 7 October 2009 Virology Journal 2009 6 158 doi 1743-422X-6-158 This article is available from http content 6 1 158 2009 Upadhyay et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Avian influenza viruses belonging to the family Orthomyxoviridae possess distinct combinations of hemagglutinin H and the neuraminidase N surface glycoproteins. Typing of both H and N antigens is essential for the epidemiological and surveillance studies. Therefore it is important to find a rapid sensitive and specific method for their assay and ELISA can be useful for this purpose by using recombinant proteins. Results The nucleoprotein NP and truncated neuraminidase subtype 3 and 7 of avian influenza virus AIV were expressed in Saccharomyces cerevisiae and used to develop an indirect enzyme-linked immunosorbent assay for antibody detection. The developed assays were evaluated with a panel of 64 chicken serum samples. The performance of NP-ELISA was compared with the commercially available ProFlok AIV ELISA kit. The results showed comparable .
